Re: [ccp4bb] problem in transformation of pqe 30 clone

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Charge vs. pH plot Isoelectric titration curve ascii plotter
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2009-05-04

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Subject: Re: problem in transformation of pqe 30 clone
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2009-05-04

Subject: Re: problem in transformation of pqe 30 clone
From: Ho-Leung Ng holeung {- at -} BERKELEY {- dot -} EDU
Date: 2009-05-04

Hello Artem,

We express almost all our proteins in BL21 derivatives. It sounds
like you've worked with many proteins that express/behave better in
XL1-Blue?

ho
UC Berkeley

-------------------------------------------------------------------------------------------------
XL1-Blue is a strain of E. coli. Whether it is or isn't an expression host
depends on the definition, and I am not going to argue semantics.

The T5 promoter works with regular garden variety RNApol of E. coli.
Therefore ANY E. coli strain is an 'expression host' for vectors that
contain this promoter.

I've expressed many proteins in XL1-Blue and I see no reason why you can't
express yours, either.

Artem

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Charge vs. pH plot Isoelectric titration curve ascii plotter
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2009-05-04

Next message:
Subject: Re: problem in transformation of pqe 30 clone
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2009-05-04