Re: [ccp4bb] Refolding of Denatured Protein

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refolding of Denatured Protein
From: David Cobessi David {- dot -} Cobessi {- at -} IBS {- dot -} FR
Date: 2009-05-05

Next message:
Subject: Re: Refolding of Denatured Protein
From: Shao-Yang Ku s {- dot -} ku {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-05-05

Subject: Re: Refolding of Denatured Protein
From: Sanjiv Kumar sanjivigib {- at -} GMAIL {- dot -} COM
Date: 2009-05-05

This protein does not have any trans membrane helices, as predicted by TMHMM
server (http://www.cbs.dtu.dk/services/TMHMM/). Protein is predicted to be
outside the cell by TMHMM. This protein is not there in the soluble
fraction, but it is in the cell pellet. I will try purification with the
detergents. Hope this works. Thanks a lot for help.

On Tue, May 5, 2009 at 6:08 PM, vinothkumar wrote:

> If it is really a membrane protein I am afraid you have to use detergent
> for purification. Since you did not use detergent the protein did not elute
> of the column, the first thing to try is to solubilize your protein in
> detergent (that is from membranes) and purify in presence of detergent. This
> way you don't have to worry about refolding. How many TM helices does your
> protein has?
>
>
> Vinothkumar K.R.
> Structural Studies Division
> MRC-Laboratory of Molecular Biology
> Hills Road, Cambridge.
> CB2 0QH
>
> Tel: 44-1223-402405
>
>
>
>
>
>

--

Sanjiv Kumar
Lab. No. 411,
Functional Genomics Unit,
Institute of Genomics and Integrative Biology,
New Delhi-110007
India

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Refolding of Denatured Protein
From: David Cobessi David {- dot -} Cobessi {- at -} IBS {- dot -} FR
Date: 2009-05-05

Next message:
Subject: Re: Refolding of Denatured Protein
From: Shao-Yang Ku s {- dot -} ku {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-05-05