Re: [ccp4bb] Lost my protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

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   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: failed compilation for Pointless
From: James Foadi james_foadi {- at -} YAHOO {- dot -} CO {- dot -} UK
Date: 2009-05-05

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Subject: Re: Lost my protein
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2009-05-05

Hi Yanming,

you can try adding 1 M of urea, 1% detergent (OG,DM) that often helps to keep proteins in solution.

Best wishes

Kornelius

On Tue, 5 May 2009 09:06:09 -0700
yanming Zhang wrote:
> Hi all,
>
> during concentrating my protein, using Amicon Ultra centrifugal filter devices, 5000g, 4C, I lost large amount of my protein (75%). I heard the same story from one of my colleagues too. It seems the membrane of the Amicon tube ate my protein.
> Why this happen and how to recover my protein?
>
> Thank you very much.
>
> Yan
>
>
>
>

----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: failed compilation for Pointless
From: James Foadi james_foadi {- at -} YAHOO {- dot -} CO {- dot -} UK
Date: 2009-05-05

Next message:
Subject: Ecoli K12 SB536
From: aka akaka druida236 {- at -} HOTMAIL {- dot -} COM
Date: 2009-05-05