[ccp4bb] His-His H-bond

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Crystallization Course: focus on membrane proteins 2007 - Last days to register
From: "Stojanoff, Vivian" stojanof {- at -} BNL {- dot -} GOV
Date: 2007-05-23

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Subject: Re: His-His H-bond
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-23

Subject: His-His H-bond
From: "david lawson (JIC)" david {- dot -} lawson {- at -} BBSRC {- dot -} AC {- dot -} UK
Date: 2007-05-23

Dear All,

We have solved a structure that has an intersubunit His-His H-bond
(ND1---NE2). The protein most likely undergoes conformational changes
that involve the making and breaking of H-bonds at this interface. In
all the protein structures I have previously studied I don't recall ever
seeing such a bond - His-His ring stacking yes, but never H-bonding. I
was wondering if anyone else had seen this type of interaction and
whether it had any functional significance. The crystals were grown at
pH 5.6.

Many thanks

Dave Lawson

-------------------------------

Dr. David M. Lawson
Biological Chemistry Dept.,
John Innes Centre,
Norwich,
NR4 7UH, UK.
Tel: +44-(0)1603-450725
Fax: +44-(0)1603-450018
Email: david.lawson@bbsrc.ac.uk
Web: http://www.jic.bbsrc.ac.uk/staff/david-lawson/index.htm

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Crystallization Course: focus on membrane proteins 2007 - Last days to register
From: "Stojanoff, Vivian" stojanof {- at -} BNL {- dot -} GOV
Date: 2007-05-23

Next message:
Subject: Re: His-His H-bond
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-23