Re: [ccp4bb] His-His H-bond

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: His-His H-bond
From: "david lawson (JIC)" david {- dot -} lawson {- at -} BBSRC {- dot -} AC {- dot -} UK
Date: 2007-05-23

Next message:
Subject: Re: His-His H-bond
From: Wim Burmeister wpb {- at -} EMBL-GRENOBLE {- dot -} FR
Date: 2007-05-23

Subject: Re: His-His H-bond
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-23

Dear David,

have a look at the Ammonium Transporter structure, AmtB, PDB entries
1XQE, 1XQF by Zheng et al. or 2NMR and so on by Javelle et al. (search
for AmtB on www.pdb.org). We've seen such an unusual shared hydrogen
bond between two histidines in the middle of the ammonia channel.

Best regards,

Dirk.

david lawson (JIC) wrote:
> Dear All,
>
> We have solved a structure that has an intersubunit His-His H-bond
> (ND1---NE2). The protein most likely undergoes conformational changes
> that involve the making and breaking of H-bonds at this interface. In
> all the protein structures I have previously studied I don't recall ever
> seeing such a bond - His-His ring stacking yes, but never H-bonding. I
> was wondering if anyone else had seen this type of interaction and
> whether it had any functional significance. The crystals were grown at
> pH 5.6.
>
> Many thanks
>
> Dave Lawson
>
> -------------------------------
>
> Dr. David M. Lawson
> Biological Chemistry Dept.,
> John Innes Centre,
> Norwich,
> NR4 7UH, UK.
> Tel: +44-(0)1603-450725
> Fax: +44-(0)1603-450018
> Email: david.lawson@bbsrc.ac.uk
> Web: http://www.jic.bbsrc.ac.uk/staff/david-lawson/index.htm
>

--

****************************************
Dirk Kostrewa
Paul Scherrer Institut
Biomolecular Research, OFLC/110
CH-5232 Villigen PSI, Switzerland
Phone: +41-56-310-4722
Fax: +41-56-310-5288
E-mail: dirk.kostrewa@psi.ch
http://sb.web.psi.ch
****************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: His-His H-bond
From: "david lawson (JIC)" david {- dot -} lawson {- at -} BBSRC {- dot -} AC {- dot -} UK
Date: 2007-05-23

Next message:
Subject: Re: His-His H-bond
From: Wim Burmeister wpb {- at -} EMBL-GRENOBLE {- dot -} FR
Date: 2007-05-23