Re: [ccp4bb] His-His H-bond

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: His-His H-bond
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-23

Next message:
Subject: Re: His-His H-bond
From: "V {- dot -} S {- dot -} Gowri" gowri {- at -} MBU {- dot -} IISC {- dot -} ERNET {- dot -} IN
Date: 2007-05-23

Subject: Re: His-His H-bond
From: Wim Burmeister wpb {- at -} EMBL-GRENOBLE {- dot -} FR
Date: 2007-05-23

david lawson (JIC) wrote:
> Dear All,
>
> We have solved a structure that has an intersubunit His-His H-bond
> (ND1---NE2). The protein most likely undergoes conformational changes
> that involve the making and breaking of H-bonds at this interface. In
> all the protein structures I have previously studied I don't recall ever
> seeing such a bond - His-His ring stacking yes, but never H-bonding. I
> was wondering if anyone else had seen this type of interaction and
> whether it had any functional significance. The crystals were grown at
> pH 5.6.
>
> Many thanks
>
> Dave Lawson
>
> -------------------------------
>
> Dr. David M. Lawson
> Biological Chemistry Dept.,
> John Innes Centre,
> Norwich,
> NR4 7UH, UK.
> Tel: +44-(0)1603-450725
> Fax: +44-(0)1603-450018
> Email: david.lawson@bbsrc.ac.uk
> Web: http://www.jic.bbsrc.ac.uk/staff/david-lawson/index.htm
>
>
>
>
>
Dear David,

why not, as long as the histidine residues are not fully protonated, and
at pH 5.6 this is still possible, the nitrogen atoms act as hydrogen
bond donors or acceptors. Look for example in pdb 1FL1 KSHV protease,
where there is a catalytic triad Ser114-His46-His134.

Yours

Wim Burmeister

--
*******************************************************************************
Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Unit of Virus Host Cell Interactions (UVHCI) UMR5233 UJF-EMBL-CNRS
6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9 FRANCE
E-mail: wpb@embl-grenoble.fr
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http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: His-His H-bond
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-23

Next message:
Subject: Re: His-His H-bond
From: "V {- dot -} S {- dot -} Gowri" gowri {- at -} MBU {- dot -} IISC {- dot -} ERNET {- dot -} IN
Date: 2007-05-23