[ccp4bb] Na/K Phosphate

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Senior Scientific Programmer Opening
From: Kim Henrick henrick {- at -} EBI {- dot -} AC {- dot -} UK
Date: 2009-05-14

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Subject: Phasing at Low Resolution
From: Clemens Grimm Clemens {- dot -} Grimm {- at -} BIOZENTRUM {- dot -} UNI-WUERZBURG {- dot -} DE
Date: 2009-05-14

Subject: Na/K Phosphate
From: Geoffrey Kwai Wai Kong geoffreykong2 {- at -} GMAIL {- dot -} COM
Date: 2009-05-14

Dear all,

Quite a few crystallisation conditions in the screens feature
'sodium/potassium phosphate'. I'm curious to know why such a Na/K mix
is there. As the pH is mostly determined by the (H2PO4)- to (HPO4)2-
ratio, is there such a need to have both cations? If so, is the Na to
K ratio important?

On the more practical side of things, if you want to explore an
initial hit based on sodium/potassium phosphate, how would you go
about it - e.g. what phosphate buffers would you make and at what
ratio would you mix them?

Thanks in advance,
Geoffrey Kong

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Senior Scientific Programmer Opening
From: Kim Henrick henrick {- at -} EBI {- dot -} AC {- dot -} UK
Date: 2009-05-14

Next message:
Subject: Phasing at Low Resolution
From: Clemens Grimm Clemens {- dot -} Grimm {- at -} BIOZENTRUM {- dot -} UNI-WUERZBURG {- dot -} DE
Date: 2009-05-14