Re: [ccp4bb] Phasing at Low Resolution

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystallizing Fluorescent Molecules
From: "V {- dot -} Nagarajan" tatoosh {- at -} GMAIL {- dot -} COM
Date: 2009-05-14

Next message:
Subject: Re: Phasing at Low Resolution
From: "J {- dot -} Preben Morth" premo {- at -} BIOXRAY {- dot -} AU {- dot -} DK
Date: 2009-05-14

Subject: Re: Phasing at Low Resolution
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2009-05-14

Regarding question 1, see:

Acta Cryst. (2008). D64, 354-367

Towards a rational approach for heavy-atom derivative screening in
protein crystallography

href="http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Agniswamy,%20J.">J.
Agniswamy, href="http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Joyce,%20M.G.">M.
G. Joyce, href="http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Hammer,%20C.H.">C.
H. Hammer and href="http://scripts.iucr.org/cgi-bin/citedin?search_on=name&author_name=Sun,%20P.D.">P.
D. Sun

Cheers.

riya doreen wrote:
cite="mid:bdb7f21a0905141241s771e4441h4de15cd630856037@mail.gmail.com"
type="cite">

I have two questions regarding derivatization:

(i) Is there a pH dependence to making derivatives ? i.e.
effectiveness of getting derivatives at low vs high pH ?

(ii) Any thoughts on the effectiveness of Barium for phasing low
resolution structures (especially for magnesium binding proteins ) ???

Thanks

Roger S. Rowlett

Professor

Colgate University Presidential Scholar

Department of Chemistry

Colgate University

13 Oak Drive

Hamilton, NY 13346

tel: (315)-228-7245

ofc: (315)-228-7395

fax: (315)-228-7935

email: rrowlett@mail.colgate.edu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystallizing Fluorescent Molecules
From: "V {- dot -} Nagarajan" tatoosh {- at -} GMAIL {- dot -} COM
Date: 2009-05-14

Next message:
Subject: Re: Phasing at Low Resolution
From: "J {- dot -} Preben Morth" premo {- at -} BIOXRAY {- dot -} AU {- dot -} DK
Date: 2009-05-14

Re: [ccp4bb] Phasing at Low Resolution

Main steps:

Special:

Basic tutorials:

Xtal community:

CCP4bb navigation

Towards a rational approach for heavy-atom derivative screening inprotein crystallography

CCP4bb navigation

Towards a rational approach for heavy-atom derivative screening in
protein crystallography