Re: [ccp4bb] Tips on fitting poorly defined loop regions

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Date: 2009-05-20

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From: Seema Mittal seema {- dot -} mittal {- at -} UMASSMED {- dot -} EDU
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Subject: Re: Tips on fitting poorly defined loop regions
From: Andrew Waight Andrew {- dot -} Waight {- at -} MED {- dot -} NYU {- dot -} EDU
Date: 2009-05-20

Thanks for these suggestions. The protein is purified and crystallized in
the presence of substrate at all times. A reason that I'd like to assign all
the polypeptide density is that the remaining density in the active site
could be my substrate. I am fairly certain that the loops do not obey the
NCS rules, especially because the best resolved of the five makes a crystal
contact while the others do not. I am running the xpleo program locally
right now, I'll let everyone know if it works out. I will also try difining
my TLS regions properly.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Post-doc positions at the Netherlands Cancer Institute (NKI)
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2009-05-20

Next message:
Subject: issues with TLS refinement
From: Seema Mittal seema {- dot -} mittal {- at -} UMASSMED {- dot -} EDU
Date: 2009-05-20