[ccp4bb] CD spectroscopy sensitivity

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Problem with Rfree and Rfac
From: Navdeep Sidhu navdeep {- dot -} sidhu {- at -} USASK {- dot -} CA
Date: 2009-05-21

Next message:
Subject: Re: CD spectroscopy sensitivity
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-05-21

Subject: CD spectroscopy sensitivity
From: "Francisco J {- dot -} Enguita" fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT
Date: 2009-05-21

Dear All

I got recently a comment on a paper review suggesting me to perform CD
spectroscopy to determine the alpha-helical content of a mutant protein
involved in a human disease. The diferences between the wild type and
the mutant protein are just 17 aminoacids. My question is if CD
spectroscopy could detect secondary structure differences between the
wild-type and the mutant proteins based on this aminoacid difference.
Let's say, is it possible to detect by CD the presence or absence of a
17 aa helix in a protein of 400 aa.

I would appreciate your opinion

All the best

Francisco J. Enguita
ITQB
Oeiras
Portugal

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Problem with Rfree and Rfac
From: Navdeep Sidhu navdeep {- dot -} sidhu {- at -} USASK {- dot -} CA
Date: 2009-05-21

Next message:
Subject: Re: CD spectroscopy sensitivity
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-05-21