[ccp4bb] another quick question...

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: new version of MR pipeline BALBES
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-24

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Subject: experiences on fluidigm topaz system
From: Tommi Kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2007-05-24

Subject: another quick question...
From: James Pauff pauffjm {- at -} YAHOO {- dot -} COM
Date: 2007-05-24

Good day all,

In COOT, when I view my coordinate pdb file with my
ccp4 map file (using "Auto-open mtz"), I have a couple
residues for which only a part of the peptide chain is
visible. E.g. for a Lys, only the terminal nitrogens
at the end of the R-group chain are visible as bonds.
The other parts of the residue (and even parts of
adjacent residues) are visible only as stars/stellate
points that may or may not be within my blue electron
density. If I click on the stars, the atom names that
appear are consistent with the missing peptide chain,
and correspond to the "missing" amino acid that has
the occassional visible R group.

Just wondering what this means, that I am seeing
stellate points that seem to be acting as place
holders for my residues in a couple locations in my
structure. The structure was obtained using molrep.

Thank you,
James Pauff

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: new version of MR pipeline BALBES
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-24

Next message:
Subject: experiences on fluidigm topaz system
From: Tommi Kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2007-05-24