[ccp4bb] removal of sulfate ion from the active site

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Maps look different from auto-mtz vs EDS vs FFT in Coot or CCP4MG.
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2007-05-24

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Subject: Re: Maps look different from auto-mtz vs EDS vs FFT in Coot or CCP4MG.
From: Joao Dias jmdias {- at -} SCRIPPS {- dot -} EDU
Date: 2007-05-24

Subject: removal of sulfate ion from the active site
From: Yue Li yl392 {- at -} CORNELL {- dot -} EDU
Date: 2007-05-24

Hi all,

I have crystals of the apo enzyme growing from 1.7M ammonium sulfate
condition. After soaking with 10mM ligand (substrate analog) which has a
phosphate group, the ligand did not enter the active site of the enzyme
because of the competition of sulfate ion and phosphate group. So I am
wondering if anyone knows how to get rid of ammonium sulfate from
crystal before soaking it with the ligand solution? Thanks a lot.

Simon

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Maps look different from auto-mtz vs EDS vs FFT in Coot or CCP4MG.
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2007-05-24

Next message:
Subject: Re: Maps look different from auto-mtz vs EDS vs FFT in Coot or CCP4MG.
From: Joao Dias jmdias {- at -} SCRIPPS {- dot -} EDU
Date: 2007-05-24