Re: [ccp4bb] BioRad CFX96 for thermofluor/DSF?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: coupling between occupancy and b-values in refinement
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2009-05-31

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Subject: Re: coupling between occupancy and b-values in refinement
From: John Badger jbadger1 {- at -} SAN {- dot -} RR {- dot -} COM
Date: 2009-05-31

Subject: Re: BioRad CFX96 for thermofluor/DSF?
From: Jürgen_Bosch jubosch {- at -} JHSPH {- dot -} EDU
Date: 2009-05-31

Sorry Charlie for the late reply.
I'm using the CFX96 5 channel with Sypro and other dyes. I tested the
machine against the Eppendorf product nd decided to go for the Biorad
version as running identical samples gave better results on the
Biorad. This could be a machine specific problem but on a 96 well
plate the detection level was ~1.5x better, you can use the HEX
channel for detection.

Jürgen

On 29 Apr 2009, at 03:06, Charlie Bond wrote:

> Hi Folks,
>
> Has anyone here had any success running a thermofluor (differential
> scanning fluorimetry) experiment on a BioRad CFX96 with SYPRO orange
> as
> the fluorophore?
>
> Our local 'technical expert' tells us it should work and gave us some
> settings to test, but was very sketchy about any detail, and in a test
> we ran we got some strange results (every well that contains SYPRO
> orange - even those with no protein - have a peak in fluorescence at
> 58oC).
>
> Before we tinker, or go and persuade a friendly molbiol lab to let us
> play with the filters in their iq5, I thought I'd see if anyone here
> had
> any advice.
>
> I'll summarise any responses I get.
>
> Cheers,
> Charlie
>
> --
> Charlie Bond
> Professorial Fellow
> University of Western Australia
> School of Biomedical, Biomolecular and Chemical Sciences
> M310
> 35 Stirling Highway
> Crawley WA 6009
> Australia
> Charles.Bond@uwa.edu.au
> +61 8 6488 4406

-
Jürgen Bosch
Johns Hopkins Bloomberg School of Public Health
Biochemistry and Molecular Biology, W8708
615 North Wolfe Street
Baltimore, MD 21205
Phone: +1-410-614-4742
Lab: +1-410-614-4894
Fax: +1-410-955-3655
http://web.me.com/bosch_lab/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: coupling between occupancy and b-values in refinement
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2009-05-31

Next message:
Subject: Re: coupling between occupancy and b-values in refinement
From: John Badger jbadger1 {- at -} SAN {- dot -} RR {- dot -} COM
Date: 2009-05-31