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[ccp4bb] AW: [ccp4bb] removal of sulfate ion from the active site
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Maps look different from auto-mtz vs EDS vs FFT in Coot or CCP4MG.
From: Joao Dias jmdias {- at -} SCRIPPS {- dot -} EDU
Date: 2007-05-24		Next message:
Subject: Re: removal of sulfate ion from the active site
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-25


Subject: AW: removal of sulfate ion from the active site
From: Herman {- dot -} Schreuder {- at -} SANOFI-AVENTIS {- dot -} COM Herman {- dot -} Schreuder {- at -} SANOFI-AVENTIS {- dot -} COM
Date: 2007-05-25

Dear Simon,

A long time ago, we have transferred crystals from a concentrated ammonium sulfate liquor to a PEG solution by first determining the equivalent PEG concentration and then essentially dunking the crystal in. It has been described in the following publication:

J. Appl. Cryst. (1988). 21, 426-429

Best regards and good luck!
Herman Schreuder


-----Ursprüngliche Nachricht-----
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Yue Li
Gesendet: Donnerstag, 24. Mai 2007 20:25
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] removal of sulfate ion from the active site

Hi all,

I have crystals of the apo enzyme growing from 1.7M ammonium sulfate condition. After soaking with 10mM ligand (substrate analog) which has a phosphate group, the ligand did not enter the active site of the enzyme because of the competition of sulfate ion and phosphate group. So I am wondering if anyone knows how to get rid of ammonium sulfate from crystal before soaking it with the ligand solution? Thanks a lot.

Simon

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Maps look different from auto-mtz vs EDS vs FFT in Coot or CCP4MG.
From: Joao Dias jmdias {- at -} SCRIPPS {- dot -} EDU
Date: 2007-05-24		Next message:
Subject: Re: removal of sulfate ion from the active site
From: Dirk Kostrewa dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-05-25
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