[ccp4bb] solvent mask with refmac

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: 3D LCD and glasses
From: "Bernhard C {- dot -} Lohkamp" bernhard {- at -} CHEM {- dot -} GLA {- dot -} AC {- dot -} UK
Date: 2009-06-02

Next message:
Subject: Postdoctoral position at Karolinska Institutet
From: Jodie Guy Jodie {- dot -} Guy {- at -} KI {- dot -} SE
Date: 2009-06-02

Subject: solvent mask with refmac
From: Ana-Maria Goncalves ana_maria {- dot -} goncalves {- at -} ESRF {- dot -} FR
Date: 2009-06-02

Dear BB,

I'm refining a structure which presents a big hydrophobic cavity where a
strong residual difference negative density can be seen. I presume that
this is an effect of not having a proper solvent mask determined and I
was wondering if there is a way to provide a better mask description to
REFMAC. The resolution of the data is 1.6 A and I have already tried
changing from simple to Babinet scaling, without any improvement. Any
advice/suggestion would be very welcome.

Many thanks,
Ana-Maria

--
Ana-Maria Goncalves
Macromolecular Crystallography Group
European Synchrotron Radiation Facility
B.P. 220, 6 rue Jules Horowitz
F-38043 GRENOBLE CEDEX
FRANCE

Tel: +33 (0) 4.76.88.19.12
Fax: +33 (0) 4.76.88.29.04

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: 3D LCD and glasses
From: "Bernhard C {- dot -} Lohkamp" bernhard {- at -} CHEM {- dot -} GLA {- dot -} AC {- dot -} UK
Date: 2009-06-02

Next message:
Subject: Postdoctoral position at Karolinska Institutet
From: Jodie Guy Jodie {- dot -} Guy {- at -} KI {- dot -} SE
Date: 2009-06-02