Re: [ccp4bb] Antwort: [ccp4bb] removal of sulfate ion from the active site

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Antwort: removal of sulfate ion from the active site
From: Djordje {- dot -} Musil {- at -} MERCK {- dot -} DE Djordje {- dot -} Musil {- at -} MERCK {- dot -} DE
Date: 2007-05-25

Next message:
Subject: Re: removal of sulfate ion from the active site
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2007-05-25

Subject: Re: Antwort: removal of sulfate ion from the active site
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-05-25

> -----Original Message-----
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On
> Behalf Of Djordje.Musil@MERCK.DE
> Sent: 25 May 2007 10:58
>
>
> One solution would be to do as Herman has suggested. But if
> your crystals do not survive for any reason (sulfate ion may
> be needed for stuctural stability of the crystal) you may
> try to cross-link your crystals using glutaraldehyde.

Does anybody happen to know how much glutaraldehyde really helps? I know
the advice well, presumably it's backed up by solid anecdotes?

Reason I ask: Cross-linking may keep the crystal aesthetically pleasing,
but does that help for the cell-to-cell variation that osmotic shock
induces?

phx.

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: removal of sulfate ion from the active site
From: "R {- dot -} M {- dot -} Garavito" garavito {- at -} MSU {- dot -} EDU
Date: 2007-05-25