[ccp4bb] deglycosylation of proteins expressed in mammalian cells at room temperature or lower

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Request recommendation for the peptide synthesis company
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Date: 2009-06-02

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From: Jan Abendroth jan {- dot -} abendroth {- at -} GMAIL {- dot -} COM
Date: 2009-06-02

Subject: deglycosylation of proteins expressed in mammalian cells at room temperature or lower
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2009-06-02

Dear All:

Recent we expressed one protein using mammalian cells but this protein was highly glycosylated (30% of the total molecular weight).

We want to remove some carbohydrate by enzymatic digestion for the convenience of crystallization. However, this protein tends to aggregate at 37 degree.

I am not sure whether EndoH and PNGase can work at 4 degree.

Can anyone give some suggestion?

Thanks a lot,

Jerry McCully

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Request recommendation for the peptide synthesis company
From: Phoebe Rice price {- at -} UCHICAGO {- dot -} EDU
Date: 2009-06-02

Next message:
Subject: ccp4i problem - bad marshal data
From: Jan Abendroth jan {- dot -} abendroth {- at -} GMAIL {- dot -} COM
Date: 2009-06-02