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Re: [ccp4bb] deglycosylation of proteins expressed in mammalian cells at room temperature or lower
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: precipitation of the protein in crystallisation solution
From: Matthew Alan Bratkowski mab259 {- at -} CORNELL {- dot -} EDU
Date: 2009-06-02		Next message:
Subject: Official PDB Nomenclature?
From: Dirk Kostrewa kostrewa {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2009-06-03


Subject: Re: deglycosylation of proteins expressed in mammalian cells at room temperature or lower
From: Pius Padayatti ppadayatti {- at -} GMAIL {- dot -} COM
Date: 2009-06-02

will work fine but leave overnight.
psp

On Tue, Jun 2, 2009 at 5:36 PM, Jerry McCully
wrote:
> Dear All:
>
>        Recent we expressed one protein using mammalian cells but this
> protein was highly glycosylated (30% of the total molecular weight).
>
>        We want to remove some carbohydrate by enzymatic digestion for the
> convenience of crystallization. However, this protein tends to aggregate at
> 37 degree.
>
>         I am not sure whether EndoH and PNGase can work at 4 degree.
>
>         Can anyone give some suggestion?
>
>         Thanks a lot,
>
> Jerry McCully
>
>
>
>
>
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--
Pius S Padayatti
Scientist,
Polgenix, Inc.
11000 Cedar Ave, Suite 260
Cleveland, OH 44106
Phone: 216-658-4528
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