Re: [ccp4bb] TEV nucleotude sequence with restriction site

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: TEV nucleotude sequence with restriction site
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-06-05

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Subject: Re: TEV nucleotude sequence with restriction site
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2009-06-05

Subject: Re: TEV nucleotude sequence with restriction site
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2009-06-05

You seem to be describing the MCS found in many TEV-site-containing
expression plasmids (am I missing something?) E.g., look at the
sequences in Sheffield et al., Protein Expression and Purification
15, 34 –39 (1999) (let me know if you want a PDF, I don't want to
send it to the whole bb)

On 5 Jun 2009, at 5:41 PM, Jacob Keller wrote:

> Dear Crystallographers,
>
> Does anybody have a TEV-protease-site-coding nucleotide sequence
> with a commonly-used restriction site in it, preferably right at
> the end? Alternatively, does some somebody know of a program to
> determine all equivalent codon permutations for a small coding
> region, filtered for resulting restriction site possibilities? It
> seems like it would be an easy enough script to write...
>
> (I have already done some googling around for such a program, with
> not much luck.)
>
> Jacob
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************

------------------------------------------------------------------------
---------------
Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA

(215) 762-7706
pat.loll@drexelmed.edu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: TEV nucleotude sequence with restriction site
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-06-05

Next message:
Subject: Re: TEV nucleotude sequence with restriction site
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2009-06-05