Re: [ccp4bb] TEV nucleotude sequence with restriction site

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: TEV nucleotude sequence with restriction site
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2009-06-05

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Subject: Re: TEV nucleotude sequence with restriction site
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-06-05

Subject: Re: TEV nucleotude sequence with restriction site
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2009-06-05

I'm not quite sure what you want, but I have a series of vectors
encoding various N-terminal tags and fusions, all followed by a TEV
site. They have an MCS standard to many pET vectors. Therefore, they
are designed to clone your gene in using the NdeI site at the 5' end
(which will, after proteolysis, leave you with GH at the N-terminus of
your protein). Other restriction enzymes in the MCS can be used, but
more amino acids will be left at your N-terminus.

I've used WatCut (from the U. Waterloo) for the silent mutagenesis
question: http://watcut.uwaterloo.ca/watcut/watcut/template.php

Best,

Cynthia

On Jun 5, 2009, at 5:41 PM, Jacob Keller wrote:

> Dear Crystallographers,
>
> Does anybody have a TEV-protease-site-coding nucleotide sequence
> with a commonly-used restriction site in it, preferably right at the
> end? Alternatively, does some somebody know of a program to
> determine all equivalent codon permutations for a small coding
> region, filtered for resulting restriction site possibilities? It
> seems like it would be an easy enough script to write...
>
> (I have already done some googling around for such a program, with
> not much luck.)
>
> Jacob
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: TEV nucleotude sequence with restriction site
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2009-06-05

Next message:
Subject: Re: TEV nucleotude sequence with restriction site
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-06-05