Re: [ccp4bb] Disordered active sites

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Disordered active sites
From: Nian Huang huangnian {- at -} GMAIL {- dot -} COM
Date: 2007-05-25

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From: Jan Abendroth janabend {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-05-25

Subject: Re: Disordered active sites
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-05-25

This is very protein-specific, for some proteins it is better to
co-crystallize with an inhibitor, then countersoak with a different
inhibitor, yet for others it is better to co-crystallize with an inhibitor
of interest directly. For all it's worth, I personally am a proponent of
the second approach, since soaking can and does generate scary artifacts.

Artem

> Hi, all,
> I met some crystal structures with disordered active sites. Soaking
> common ligands can not make it become ordered. I am wondering what
> people generally do in such situation.
>
> Thanks,
>
> Nian Huang
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Disordered active sites
From: Nian Huang huangnian {- at -} GMAIL {- dot -} COM
Date: 2007-05-25

Next message:
Subject: mosflm getbox error
From: Jan Abendroth janabend {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-05-25