[ccp4bb] Phase Comparison

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: off topic
From: Andy Torelli att29 {- at -} CORNELL {- dot -} EDU
Date: 2009-06-19

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Subject: Molecular Replacement of a small peptide
From: "Sickmier, Allen" sickmier {- at -} AMGEN {- dot -} COM
Date: 2009-06-19

Subject: Phase Comparison
From: James Tucker Swindell II jtswinde {- at -} SECSG {- dot -} UGA {- dot -} EDU
Date: 2009-06-19

I want to compare my experimental resolve phases (before final refinement)
to my model(PDB) phases.

I have tried to compare the model phases vs. experimental phases using:
Sfall (to ensure I have the appropriate PHI columns) -> Cad (for merging
mtz files) -> Phase comparison (Clipper utilities).

My space group is P42

My experimental resolve map looks great, approx 70% traced. The model has
been refined to a R-factor of 24%(Rfree 28%). So I would think the
delta-phase (phase error) would be small between the experimental
structure and model. I am finding a 90 degree phase error using the above
Sfall -> Cad -> Phase Comparison approach. I must be doing something
wrong. Any help would be greatly appreciated.

Thanks
James

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: off topic
From: Andy Torelli att29 {- at -} CORNELL {- dot -} EDU
Date: 2009-06-19

Next message:
Subject: Molecular Replacement of a small peptide
From: "Sickmier, Allen" sickmier {- at -} AMGEN {- dot -} COM
Date: 2009-06-19