Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

[ccp4bb] Problem about Molecular Replacement with identity 30% model
- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Molecular Replacement of a small peptide
From: "Mark J {- dot -} van Raaij" mark {- dot -} vanraaij {- at -} USC {- dot -} ES
Date: 2009-06-21		Next message:
Subject: problem of Refmac refinement on twinning data
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2009-06-21


Subject: Problem about Molecular Replacement with identity 30% model
From: Ëｨ sunjian00000 {- at -} GMAIL {- dot -} COM
Date: 2009-06-21

Dear all:
Recently,I have a problem about molecular replacement with
identity 30% model. There are two moleculars in AU.Through
self-rotation analyze,there are a two-fold axis in AU along X axis or
Z axis.Then I rotate and translate the model with CNS MR module.
several models have been searched according self-rotation criterion.I
build the model using Phenix and Arp/wArp,but there are no good
results.The best result is R/Rfree 0.47/0.48.Also,I do MR with Phaser
and Molrep.The Phaser indicate Z-scores 6.5 and no good
results,neither do the Molrep.I am very confused about several
questions:
1.Just 30% identity model can be used for MR model?What at
least identity should be acquired for MR?
2.Are there good program editing the model except Chainsaw?
3.How can I make sure my rotated and translated MR model is
right and can be used for next model building and refinement?


--
Best
Wishes!

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: Molecular Replacement of a small peptide
From: "Mark J {- dot -} van Raaij" mark {- dot -} vanraaij {- at -} USC {- dot -} ES
Date: 2009-06-21		Next message:
Subject: problem of Refmac refinement on twinning data
From: Jiamu Du jiamudu {- at -} GMAIL {- dot -} COM
Date: 2009-06-21
ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd