[ccp4bb] protease cleavage sites

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: DCO-X error with setup
From: Kevin Jude kjude {- at -} SAS {- dot -} UPENN {- dot -} EDU
Date: 2007-03-05

Next message:
Subject: Re: Very weird
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-05

Subject: protease cleavage sites
From: Rene Frank rf3 {- at -} SANGER {- dot -} AC {- dot -} UK
Date: 2007-03-05

Hi,

A non-ccp4 Q. Sorry.

I would like to use a cleavable purification tag at the N-terminus/
extracellular end of my membrane protein for purification. Before I
start, I wonder if someone could recommend a particular protease site
that I can engineer between the tag and my protein? How about a
proprietary cleavage system such as the PreScission protease (GE
Healthcare)? I would be grateful to hear success and horror stories
in this area.

Best wishes,

Rene

================================================
Dr R.A.W. Frank, PhD
Royal Commission for the Exhibition of 1851 Research Fellow

Prof Seth Grant Lab / Genes to Cognition
Wellcome Trust Sanger Institute
Hinxton
Cambridge CB10 1SA

Work Tel: 0044 (0)1223 834244 ext. 7318
Cell No.: 0044 (0)7870 208280
===============================================

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: DCO-X error with setup
From: Kevin Jude kjude {- at -} SAS {- dot -} UPENN {- dot -} EDU
Date: 2007-03-05

Next message:
Subject: Re: Very weird
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-05