[ccp4bb] protease cleavage sites

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2007 <-- March 2007 <-- 05 March 2007

Previous message:
Subject: DCO-X error with setup
From: Kevin Jude kjude {- at -} SAS {- dot -} UPENN {- dot -} EDU
Date: 2007-03-05

Next message:
Subject: Re: Very weird
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-05

Subject: protease cleavage sites
From: Rene Frank rf3 {- at -} SANGER {- dot -} AC {- dot -} UK
Date: 2007-03-05

Hi,

A non-ccp4 Q. Sorry.

I would like to use a cleavable purification tag at the N-terminus/
extracellular end of my membrane protein for purification. Before I
start, I wonder if someone could recommend a particular protease site
that I can engineer between the tag and my protein? How about a
proprietary cleavage system such as the PreScission protease (GE
Healthcare)? I would be grateful to hear success and horror stories
in this area.

Best wishes,

Rene

================================================
Dr R.A.W. Frank, PhD
Royal Commission for the Exhibition of 1851 Research Fellow

Prof Seth Grant Lab / Genes to Cognition
Wellcome Trust Sanger Institute
Hinxton
Cambridge CB10 1SA

Work Tel: 0044 (0)1223 834244 ext. 7318
Cell No.: 0044 (0)7870 208280
===============================================

CCP4bb navigation

CCP4bb <-- 2007 <-- March 2007 <-- 05 March 2007

Previous message:
Subject: DCO-X error with setup
From: Kevin Jude kjude {- at -} SAS {- dot -} UPENN {- dot -} EDU
Date: 2007-03-05

Next message:
Subject: Re: Very weird
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-05