Re: [ccp4bb] low UV reading on AKTA prime

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: low UV reading on AKTA prime
From: Matt Colins matt {- dot -} colins2009 {- at -} YAHOO {- dot -} COM
Date: 2009-07-01

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Subject: Re: low UV reading on AKTA prime
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2009-07-01

Subject: Re: low UV reading on AKTA prime
From: Scott Walsh walshs {- at -} UMBI {- dot -} UMD {- dot -} EDU
Date: 2009-07-01

Hi Matt,

Check to make sure the A280 filter is clean. Ours was filthy. You might need to replace this (~$350). We were experiencing the same thing you mentioned and just had the GE technician out today. Also, make sure the A280 dial is set correctly on the lamp. Please check the manual for guidance.

Best,

Scott

----- Original Message -----
From: Matt Colins
Date: Wednesday, July 1, 2009 4:40 pm
Subject: [ccp4bb] low UV reading on AKTA prime
To: CCP4BB@JISCMAIL.AC.UK

> Hi all,
>
> We recently got low UV reading on our AKTA prime. We contacted
> GE healthcare, but was told that the UV reading on AKTA prime
> should be 20% of the spectrometer reading, because the path
> length of the flow cell of AKTA prime is only 2 mm (20% of the
> pathlength of a spectrometer cuvette).
>
> I am not sure whether that is the case, but we used to get much
> higher UV readings on the same AKTA prime (almost the same as
> the spectrometer reading). The UV reading just keeps dropping
> over time in the past several months.
>
> Here I have two questions. First, should the UV reading on
> AKTA prime be 20% of the spectrometer reading? Second, what
> could go wrong with our AKTA prime? (I know it is not the lamp,
> because we put in a new lamp and it didn't solve the problem)
>
> Thanks a lot!
> Matt
>
>
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: low UV reading on AKTA prime
From: Matt Colins matt {- dot -} colins2009 {- at -} YAHOO {- dot -} COM
Date: 2009-07-01

Next message:
Subject: Re: low UV reading on AKTA prime
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2009-07-01