Re: [ccp4bb] low UV reading on AKTA prime

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: low UV reading on AKTA prime
From: Scott Walsh walshs {- at -} UMBI {- dot -} UMD {- dot -} EDU
Date: 2009-07-01

Next message:
Subject: space group R32: H settings
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2009-07-01

Subject: Re: low UV reading on AKTA prime
From: Patrick Loll pat {- dot -} loll {- at -} DREXEL {- dot -} EDU
Date: 2009-07-01

I second Scott's post. About the only problem we've had with our
Akta instruments is this type of degradation of the filter. I'm not
sure what the mechanism is, but the filters do seem to crap out after
a while, at least in the cold room (oxidation? I have no idea of what
the filter is made of...).

Pat

On 1 Jul 2009, at 5:07 PM, Scott Walsh wrote:

> Hi Matt,
>
> Check to make sure the A280 filter is clean. Ours was filthy. You
> might need to replace this (~$350). We were experiencing the same
> thing you mentioned and just had the GE technician out today.
> Also, make sure the A280 dial is set correctly on the lamp. Please
> check the manual for guidance.
>
> Best,
>
> Scott
>
> ----- Original Message -----
> From: Matt Colins
> Date: Wednesday, July 1, 2009 4:40 pm
> Subject: [ccp4bb] low UV reading on AKTA prime
> To: CCP4BB@JISCMAIL.AC.UK
>
> > Hi all,
> >
> > We recently got low UV reading on our AKTA prime. We contacted
> > GE healthcare, but was told that the UV reading on AKTA prime
> > should be 20% of the spectrometer reading, because the path
> > length of the flow cell of AKTA prime is only 2 mm (20% of the
> > pathlength of a spectrometer cuvette).
> >
> > I am not sure whether that is the case, but we used to get much
> > higher UV readings on the same AKTA prime (almost the same as
> > the spectrometer reading). The UV reading just keeps dropping
> > over time in the past several months.
> >
> > Here I have two questions. First, should the UV reading on
> > AKTA prime be 20% of the spectrometer reading? Second, what
> > could go wrong with our AKTA prime? (I know it is not the lamp,
> > because we put in a new lamp and it didn't solve the problem)
> >
> > Thanks a lot!
> > Matt
> >
> >
> >
> >

------------------------------------------------------------------------
---------------
Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA

(215) 762-7706
pat.loll@drexelmed.edu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: low UV reading on AKTA prime
From: Scott Walsh walshs {- at -} UMBI {- dot -} UMD {- dot -} EDU
Date: 2009-07-01

Next message:
Subject: space group R32: H settings
From: Jerry McCully for-crystallizaiton {- at -} HOTMAIL {- dot -} COM
Date: 2009-07-01