[ccp4bb] low UV reading on AKTA prime

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: very high concentration of protein
From: Filip Van Petegem filip {- dot -} vanpetegem {- at -} GMAIL {- dot -} COM
Date: 2009-07-02

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Subject: Re: multi-domain protein with identical tertiary structure
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2009-07-02

Subject: low UV reading on AKTA prime
From: Palm palm {- at -} UNI-GREIFSWALD {- dot -} DE
Date: 2009-07-02

Hi all,
another reason to get low readings from the UV meter can be a drift
in the baseline. The photometer is properly calibrated only when
you turn it on, I think. Since we have our machine running in the
coldroom without (usually) turning it off for month, we have observed
wrong 260/280 ratios and weak signals at 280 nm. This was simply
cured by turning off and on.
Gottfried

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: very high concentration of protein
From: Filip Van Petegem filip {- dot -} vanpetegem {- at -} GMAIL {- dot -} COM
Date: 2009-07-02

Next message:
Subject: Re: multi-domain protein with identical tertiary structure
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2009-07-02