Re: [ccp4bb] Shredded E coli pellets

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: multi-domain protein with identical tertiary structure
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2009-07-02

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Subject: Re: Shredded E coli pellets
From: "Mark J {- dot -} van Raaij" mark {- dot -} vanraaij {- at -} USC {- dot -} ES
Date: 2009-07-02

Subject: Re: Shredded E coli pellets
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-02

Okay, it seems that the consensus is phage infection. Is there anything to
seal the diagnosis? Also, does anybody have literature on de-phaging
glassware? I am assuming that regular autoclaving will not do the trick?

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

----- Original Message -----
From: "Jacob Keller"
To:
Sent: Thursday, July 02, 2009 11:42 AM
Subject: [ccp4bb] Shredded E coli pellets

> Dear crystallographers,
>
> I recently expressed some new constructs, and found after my usual
> expression protocol that the cell pellets were not compacted at the bottom
> corner of the bottles us usual, but were instead smeared as a film on the
> side, and further, were somewhat clumpy, like clots, and with a smaller
> pellet in the usual location. The centrifugation was exactly as usual. I
> noticed that there was also a bit more foam in the medium than usual, but
> I am not convinced that this was the issue, although it might be a
> symptom. My suspicion is that the constructs are lethal and cause cell
> lysis, but I am not sure. Has anybody seen this phenomenon before, and
> gotten to the bottom of it?
>
> Jacob Keller
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: multi-domain protein with identical tertiary structure
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2009-07-02

Next message:
Subject: Re: Shredded E coli pellets
From: "Mark J {- dot -} van Raaij" mark {- dot -} vanraaij {- at -} USC {- dot -} ES
Date: 2009-07-02