Re: [ccp4bb] can I try crystallization in high salt?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: "Stein, ND (Norman)" norman {- dot -} stein {- at -} STFC {- dot -} AC {- dot -} UK
Date: 2009-07-03

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From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2009-07-03

Subject: Re: can I try crystallization in high salt?
From: Djordje Francuski francuz {- at -} CHEMIE {- dot -} FU-BERLIN {- dot -} DE
Date: 2009-07-03

In short, yes you can. Although it is often avoided due to the limitations
that high salt samples may have, you still have a good chance to
crystallize the protein in the original high salt buffer. In my case a
small alcohol was the best precipitating agent. As with most proteins, try
it, try it, try it. First find the correct buffer condition where your
protein is stable and monomodal (DLS is a good tool to check this), and
then start screening.

Good luck,
Djordje

> Dear All,
> I have a peri domain protein that is stable in high salt concentration(500
> mM), if I dialysis to a lower salt buffer and then concentrate, it'll
> preticipate out. If I need to crystallize it, can I use the high salt
> buffer? Is there any optimization kits that could help to increase the
> solubility? Thanks.
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Equipment
From: "Stein, ND (Norman)" norman {- dot -} stein {- at -} STFC {- dot -} AC {- dot -} UK
Date: 2009-07-03

Next message:
Subject: CCP4 / Ubuntu 9.04 64-bit
From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2009-07-03