[ccp4bb] Why NCS doesn't help?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: CCP4 / Ubuntu 9.04 64-bit
From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2009-07-03

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Subject: Re: How to express a >95KD FAD protein
From: Kn Ly kn {- dot -} ly {- at -} AUCKLAND {- dot -} AC {- dot -} NZ
Date: 2009-07-04

Subject: Why NCS doesn't help?
From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
Date: 2009-07-03

Dear All,

In refining my structure (two molecules in au) that was solved with molecular replacement, refinement starting with restrained lowered the R-free to about 0.35 while with NCS increased the R-free to 0.55. Does it imply that two molecules are quite different or something wrong with the refinement?

Thank you very much for your suggestions?

Best wishes,

Sun Tang

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: CCP4 / Ubuntu 9.04 64-bit
From: Thomas J Magliery PhD magliery {- at -} CHEMISTRY {- dot -} OHIO-STATE {- dot -} EDU
Date: 2009-07-03

Next message:
Subject: Re: How to express a >95KD FAD protein
From: Kn Ly kn {- dot -} ly {- at -} AUCKLAND {- dot -} AC {- dot -} NZ
Date: 2009-07-04