Re: [ccp4bb] How to express a >95KD FAD protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Why NCS doesn't help?
From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
Date: 2009-07-03

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Subject: Re: CCP4 / Ubuntu 9.04 64-bit
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-07-04

Subject: Re: How to express a >95KD FAD protein
From: Kn Ly kn {- dot -} ly {- at -} AUCKLAND {- dot -} AC {- dot -} NZ
Date: 2009-07-04

try codon optimisation
use Rosetta 2 strains
fuse to MBP
I expressed a yeast protein of ~100 KDa, expression was low until I fused it
to MBp (resulting protein ~ 150 KDa)

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Why NCS doesn't help?
From: Sun Tang suntang2222 {- at -} YAHOO {- dot -} COM
Date: 2009-07-03

Next message:
Subject: Re: CCP4 / Ubuntu 9.04 64-bit
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-07-04