[ccp4bb] Bubbles on crystals

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: AstraZeneca US permanent PX position
From: "Read, Jon" Jon {- dot -} Read {- at -} ASTRAZENECA {- dot -} COM
Date: 2009-07-06

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Subject: Re: Bubbles on crystals
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2009-07-06

Subject: Bubbles on crystals
From: kumar bputcha {- at -} UTK {- dot -} EDU
Date: 2009-07-06

Dear members,
I have protein crystals grown in a condition with Isopropanol (13%) and
citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15%
Isopropanol), small bubbles appear on the surface of the crystals. These
tiny bubbles sometimes disappear in few seconds and sometimes not. Crystals
are exposed to air only for 2-3 seconds in the process of transfer. I am
worried if the formation of these bubbles is due to evaporation of
isopropanol form the crystal and if it affects the crystal lattice. These
crystals diffract X-rays upto 4 angstroms. Any ideas how to avoid this
problem?
Thank you
Kumar

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: AstraZeneca US permanent PX position
From: "Read, Jon" Jon {- dot -} Read {- at -} ASTRAZENECA {- dot -} COM
Date: 2009-07-06

Next message:
Subject: Re: Bubbles on crystals
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2009-07-06