Re: [ccp4bb] Bubbles on crystals

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: CCP4 / Ubuntu 9.04 64-bit
From: Stephen Weeks sd {- dot -} weeksy {- at -} GMAIL {- dot -} COM
Date: 2009-07-06

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Subject: Re: Bubbles on crystals
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-06

Subject: Re: Bubbles on crystals
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2009-07-06

Hi,

If you're worried - instead of transfering the crystal, just flood the
entire drop with excess of your cryo buffer. No exposure to air this way.
I bet that the bubbles won't go away though :)

Artem

> Dear members,
> I have protein crystals grown in a condition with Isopropanol (13%) and
> citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
> glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15%
> Isopropanol), small bubbles appear on the surface of the crystals. These
> tiny bubbles sometimes disappear in few seconds and sometimes not.
> Crystals
> are exposed to air only for 2-3 seconds in the process of transfer. I am
> worried if the formation of these bubbles is due to evaporation of
> isopropanol form the crystal and if it affects the crystal lattice. These
> crystals diffract X-rays upto 4 angstroms. Any ideas how to avoid this
> problem?
> Thank you
> Kumar
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: CCP4 / Ubuntu 9.04 64-bit
From: Stephen Weeks sd {- dot -} weeksy {- at -} GMAIL {- dot -} COM
Date: 2009-07-06

Next message:
Subject: Re: Bubbles on crystals
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-06