Re: [ccp4bb] Bubbles on crystals

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Bubbles on crystals
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2009-07-06

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From: Cale Dakwar c {- dot -} dakwar {- at -} GMAIL {- dot -} COM
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Subject: Re: Bubbles on crystals
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-06

Perhaps the bubbles are related to the bubbles which form when mixing
alcohols with water, e.g., when making coomassie destain? And what are those
bubbles, by the way?

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
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----- Original Message -----
From:
To:
Sent: Monday, July 06, 2009 5:56 PM
Subject: Re: [ccp4bb] Bubbles on crystals

> Hi,
>
> If you're worried - instead of transfering the crystal, just flood the
> entire drop with excess of your cryo buffer. No exposure to air this way.
> I bet that the bubbles won't go away though :)
>
> Artem
>
>> Dear members,
>> I have protein crystals grown in a condition with Isopropanol (13%) and
>> citric acid (0.2 M). When I transfer the crystals to cryo-buffer (30%
>> glycerol or 25% ethyleneglycol with 0.25 M citric acid and 13%-15%
>> Isopropanol), small bubbles appear on the surface of the crystals. These
>> tiny bubbles sometimes disappear in few seconds and sometimes not.
>> Crystals
>> are exposed to air only for 2-3 seconds in the process of transfer. I am
>> worried if the formation of these bubbles is due to evaporation of
>> isopropanol form the crystal and if it affects the crystal lattice. These
>> crystals diffract X-rays upto 4 angstroms. Any ideas how to avoid this
>> problem?
>> Thank you
>> Kumar
>>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Bubbles on crystals
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2009-07-06

Next message:
Subject: PDB Validation Server
From: Cale Dakwar c {- dot -} dakwar {- at -} GMAIL {- dot -} COM
Date: 2009-07-06