Re: [ccp4bb] Lipid content/removal

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Lipid content/removal
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2009-07-07

Next message:
Subject: Re: Lipid content/removal
From: "Clayton, Gina Martyn" gina_clayton {- at -} MERCK {- dot -} COM
Date: 2009-07-07

Subject: Re: Lipid content/removal
From: "Soisson, Stephen" stephen_soisson {- at -} MERCK {- dot -} COM
Date: 2009-07-07

Activated charcoal (see serum albumin structure references) can remove
fatty acids.

Good luck-

Steve

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jacob Keller
Sent: Tuesday, July 07, 2009 1:01 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Lipid content/removal

Dear Crystallographers,

does anybody know of a simple, fast way to determine whether a protein
sample contains lipid, and roughly to determine the quantity? As
corollary,
does anybody know of a quick way to extract/remove the putative lipid?
In my
case, I am working with an extremely robust/stable soluble protein which
may
contain bacterial lipids.

Thanks for your consideration,

Jacob Keller

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Lipid content/removal
From: "Van Den Berg, Bert" Lambertus {- dot -} VandenBerg {- at -} UMASSMED {- dot -} EDU
Date: 2009-07-07

Next message:
Subject: Re: Lipid content/removal
From: "Clayton, Gina Martyn" gina_clayton {- at -} MERCK {- dot -} COM
Date: 2009-07-07