Re: [ccp4bb] low UV reading on AKTA prime

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Jared Sampson jared {- dot -} sampson {- at -} NYUMC {- dot -} ORG
Date: 2009-07-08

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Subject: Re: low UV reading on AKTA prime
From: Marius Schmidt marius {- dot -} schmidt {- at -} PH {- dot -} TUM {- dot -} DE
Date: 2009-07-08

Yes, our new Akta prime has also only 2 mm path length
and readings are, therefore, much smaller than with
1 cm path length in a typical spectro-photometer.

Best
Marius

> Hi all,
>
> We recently got low UV reading on our AKTA prime. We contacted GE
> healthcare, but was told that the UV reading on AKTA prime should be
> 20% of the spectrometer reading, because the path length of the flow
> cell of AKTA prime is only 2 mm (20% of the pathlength of a
> spectrometer cuvette).
>
> I am not sure whether that is the case, but we used to get much
> higher UV readings on the same AKTA prime (almost the same as the
> spectrometer reading). The UV reading just keeps dropping over time
> in the past several months.
>
> Here I have two questions. First, should the UV reading on AKTA
> prime be 20% of the spectrometer reading? Second, what could go wrong
> with our AKTA prime? (I know it is not the lamp, because we put in a
> new lamp and it didn't solve the problem)
>
> Thanks a lot!
> Matt
>
>

Dr.habil. Marius Schmidt
Asst. Professor
University of Wisconsin-Milwaukee
Department of Physics Room 454
1900 E. Kenwood Blvd.
Milwaukee, WI 53211

phone: +1-414-229-4338
email: m-schmidt@uwm.edu
http://users.physik.tu-muenchen.de/marius/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: unknown density
From: Jared Sampson jared {- dot -} sampson {- at -} NYUMC {- dot -} ORG
Date: 2009-07-08

Next message:
Subject: [OT] peroxids in Triton
From: Justin Lecher j {- dot -} lecher {- at -} FZ-JUELICH {- dot -} DE
Date: 2009-07-09