Quick navigation: Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |    Advert   |   
 

Re: [ccp4bb] small-angle scattering and radiation damage control
- Protein crystallography

Main steps:

   - Protein purification
   - Crystallisation

Special:

   - Programs for crystallography
   - X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

   - CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: [ot] protein-protein interaction via fluorescence polarization anisotropy
From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2009-07-10		Next message:
Subject: Active site similarity search
From: Raja Dey deyraja1 {- at -} YAHOO {- dot -} CO {- dot -} IN
Date: 2009-07-10


Subject: Re: small-angle scattering and radiation damage control
From: Richard Gillilan reg8 {- at -} CORNELL {- dot -} EDU
Date: 2009-07-10

There was a paper a couple years ago that suggested very low
concentration of glycerol (I think 0.5%). I recently did some
systematic comparisons of different glycerol concentrations and found
that higher concentrations do seem to worsen the so-called
concentration effects. This makes intuitive sense. Adding glycerol
may effectively decrease the solubility of a protein and so the
solution behaves more like a concentrated solution with correlations
between particles in the structure factor. Only speculation at this
point of course. I also worry about glycerol effecting the oligomeric
state of a protein. Nonetheless, it seems to be a good thing in low
concentrations.

Working at 4C may help.


Richard Gillilan
MacCHESS




On Jul 9, 2009, at 8:57 PM, Susan Tsutakawa wrote:

> Hi Bill,
>
> 5-10% glycerol usually helps in the majority of cases. However,
> some proteins require a scan of different conditions and different
> protein concentrations. Like everything else, it's protein
> dependent. Changing the wavelength can also make a difference. I
> also know some SAXS beamlines like at the APS have capillary flow
> cells so they just do hit and runs.
>
> Susan
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: [ot] protein-protein interaction via fluorescence polarization anisotropy
From: Sebastiano Pasqualato sebastiano {- dot -} pasqualato {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2009-07-10		Next message:
Subject: Active site similarity search
From: Raja Dey deyraja1 {- at -} YAHOO {- dot -} CO {- dot -} IN
Date: 2009-07-10
ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd