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Re: [ccp4bb] How to determine ligand binding from diffraction pattern?
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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: A babyish question on coot
From: Yanming Zhang yzhang {- at -} MAILER {- dot -} SB {- dot -} FSU {- dot -} EDU
Date: 2007-05-29		Next message:
Subject: Re: A babyish question on coot
From: Roberto Steiner roberto {- dot -} steiner {- at -} KCL {- dot -} AC {- dot -} UK
Date: 2007-05-29


Subject: Re: How to determine ligand binding from diffraction pattern?
From: "Gerhardt, Stefan" Stefan {- dot -} Gerhardt {- at -} ASTRAZENECA {- dot -} COM
Date: 2007-05-29

Dear Joe,

Reflections that correspond to the reflection conditions of your space group symmetry (e.g. h=2n, h+k=2n, l=2n for C4) and are the reciprocal space equivalent of the ligand binding pocket in real space should be considered for comparison. But make sure you collect corresponding reflections also in lower resolution shells (ones that might fall behind the beamstop) as these will have a strong effect on results (Fourier ripples).

or it might be more straight forward to solve the ligand-bound structure ;-)

best regards

Stefan



-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]On Behalf Of Joe Chen
Sent: 28 May 2007 21:35
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] How to determine ligand binding from diffraction pattern?


Dear all,


Is there a simple way to determine whether ligand is bound or not by comparing the diffraction patterns between ligand-free (structure known) and ligand-soaked protein crystals? I would like to solve the ligand bound protein structure, but before I do so, I have to find out if the ligand is actually bound. Thank you very much!


Best,


Joe



CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999
Previous message:
Subject: Re: A babyish question on coot
From: Yanming Zhang yzhang {- at -} MAILER {- dot -} SB {- dot -} FSU {- dot -} EDU
Date: 2007-05-29		Next message:
Subject: Re: A babyish question on coot
From: Roberto Steiner roberto {- dot -} steiner {- at -} KCL {- dot -} AC {- dot -} UK
Date: 2007-05-29
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