Re: [ccp4bb] heavy atom derivative choice

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Summary IPTG suppliers (mainly Europe)
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-15

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Subject: AW: Summary IPTG suppliers (mainly Europe)
From: Clemens Steegborn Clemens {- dot -} Steegborn {- at -} RUB {- dot -} DE
Date: 2009-07-15

Subject: Re: heavy atom derivative choice
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-15

Xenon/Krypton, anyone? If you have the equipment, might as well try it. I
think I have seen the apparatus at some beamlines, although I did hear
recently that xenon is ridiculously expensive now.

Jacob Keller

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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
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Evanston IL 60208
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----- Original Message -----
From: "Phil Jeffrey"
To:
Sent: Wednesday, July 15, 2009 11:32 AM
Subject: Re: [ccp4bb] heavy atom derivative choice

>X Xiong, Cellular & Molecular Medicine wrote:
>> My Question is:
>>
>> Does mercury tends to get into the protein core to denature protein or
>> not?
>
> This is more likely to happen for a small "bare" Hg like Hg2+ in HgCl2 or
> Hg(OAc)2 than it is for a large organomercury compound like PCMB, PCMBS
> etc so if you were especially concerned about that, start with the latter
> compounds. I'd also probably try Me3Pb(OAc) as an alternative to
> mercurials.
>
> Phil Jeffrey
> Princeton
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Summary IPTG suppliers (mainly Europe)
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2009-07-15

Next message:
Subject: AW: Summary IPTG suppliers (mainly Europe)
From: Clemens Steegborn Clemens {- dot -} Steegborn {- at -} RUB {- dot -} DE
Date: 2009-07-15