Re: [ccp4bb] Disordered active sites

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: crystal shipping at room temperature
From: Mark Agacan M {- dot -} Agacan {- at -} DUNDEE {- dot -} AC {- dot -} UK
Date: 2007-05-29

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Subject: Re: How to determine ligand binding from diffraction pattern?
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-05-29

Subject: Re: Disordered active sites
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-05-29

Nian Huang wrote:
> Hi, all,
> I met some crystal structures with disordered active sites. Soaking
> common ligands can not make it become ordered. I am wondering what
> people generally do in such situation.
>
> Thanks,
>
> Nian Huang
>
>
Swear?!

This is quite common - the usual tricks may work - Sulphate ,
phosphate may bind there but it depends on your true substrate, and
often it takes a clever chemist to make a suitable substrate mimic.
Eleanor

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: crystal shipping at room temperature
From: Mark Agacan M {- dot -} Agacan {- at -} DUNDEE {- dot -} AC {- dot -} UK
Date: 2007-05-29

Next message:
Subject: Re: How to determine ligand binding from diffraction pattern?
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-05-29