[ccp4bb] About sel-met oxidation

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: maprot question
From: Peter Grey petgxray {- at -} GMAIL {- dot -} COM
Date: 2009-08-03

Next message:
Subject: Re: CCP4 / Ubuntu 9.04 64-bit
From: Stephen Weeks sd {- dot -} weeksy {- at -} GMAIL {- dot -} COM
Date: 2009-08-04

Subject: About sel-met oxidation
From: tat cheung cheng theifire {- at -} YAHOO {- dot -} COM {- dot -} HK
Date: 2009-08-03

Hi all,

I am now preparing to label my protein with selenium-methionine for phasing. As I have heard that selenium-methionine is prone to oxidation, so is it a must to add reducing agent all along the purification? if so, which reducing agent and what concentration is better ? and any alternatives besides TCEP if i am going to purify it through a Ni column?
Thanks a lot.

T.C. Cheng

Yahoo!»´ä´£¨Ñºô¤W¦w¥þ§ð²¤¡A±Ð§A¦p¦ó¨¾½d¶Â«È! ½Ð«e©¹ http://hk.promo.yahoo.com/security/ ¤F¸Ñ§ó¦h!

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: maprot question
From: Peter Grey petgxray {- at -} GMAIL {- dot -} COM
Date: 2009-08-03

Next message:
Subject: Re: CCP4 / Ubuntu 9.04 64-bit
From: Stephen Weeks sd {- dot -} weeksy {- at -} GMAIL {- dot -} COM
Date: 2009-08-04