[ccp4bb] Zinc or Iron binding protein, that is a question!

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Protein Biochemistry Positions at Merck
From: "Soisson, Stephen M" stephen_soisson {- at -} MERCK {- dot -} COM
Date: 2009-08-05

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Subject: Re: Zinc or Iron binding protein, that is a question!
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-08-06

Subject: Zinc or Iron binding protein, that is a question!
From: Xuan Yang pattisyang {- at -} GMAIL {- dot -} COM
Date: 2009-08-05

Dear Sir or Madam,

The ICP-ES results indicated that 1 molar my protein purified from E.coli
Origami(DE3) contained about a half molar Zinc and nearly a quarter
molar Iron (whether II or III was not available). The protein carried a MBP
tag on the N-terminal and the situation was similar with or without His tag
at the C terminal. I want to determine whether my protein really bind Zinc
or Iron. Does anyone have any experience about such problems?

Specifically, now I want to compare the binding efficiency on various IMAC,
i.e. 50mM ZnSO4, FeSO4, Fe2(SO4)3, NiSO4(control), or CuSO4(control).
However, considering the instability of Fe(II) in solution, the design
still seemed problematic.

Sincerely,

Xuan Yang

National Laboratory of Biomacromolecules and
Center for Infection and Immunity,
Institute of Biophysics,
Chinese Academy of Sciences,
Room 1617, 15 DaTun Road,Chaoyang District,
Beijing, China, 100101
Tel: 86-10-64884329
Academic email: YangX@moon.ibp.ac.cn
We will either find a way or make one.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Protein Biochemistry Positions at Merck
From: "Soisson, Stephen M" stephen_soisson {- at -} MERCK {- dot -} COM
Date: 2009-08-05

Next message:
Subject: Re: Zinc or Iron binding protein, that is a question!
From: Guenter Fritz guenter {- dot -} fritz {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2009-08-06