[ccp4bb] TLS restrained refinement

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Postdoctoral position at MacCHESS - CORRECTION
From: Marian Szebenyi dms35 {- at -} CORNELL {- dot -} EDU
Date: 2009-08-07

Next message:
Subject: cryoEM maps out of scale for docking experiments
From: Filip Van Petegem filip {- dot -} vanpetegem {- at -} GMAIL {- dot -} COM
Date: 2009-08-07

Subject: TLS restrained refinement
From: ManojSaxena mks131 {- at -} REDIFFMAIL {- dot -} COM
Date: 2009-08-07

Hi,

I am using Refmac 5 for refinement of my protein model (solution by molecular replacement) with TLS restrained refinement option on.Can anyone please guide me about the parameters settings for TLS.I have used defaults .Wether it is good to use multiple TLS restrained refinements rounds.I have observed that R free comes down when I use TLS restrained refinement(after coot building) but decrease is less when I use just restrained refinement. At present my Rfee =0.24 and R=.18

Thanks

Manoj

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Postdoctoral position at MacCHESS - CORRECTION
From: Marian Szebenyi dms35 {- at -} CORNELL {- dot -} EDU
Date: 2009-08-07

Next message:
Subject: cryoEM maps out of scale for docking experiments
From: Filip Van Petegem filip {- dot -} vanpetegem {- at -} GMAIL {- dot -} COM
Date: 2009-08-07