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[ccp4bb] cryoEM maps out of scale for docking experiments |
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CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: cryoEM maps out of scale for docking experiments From: Filip Van Petegem filip {- dot -} vanpetegem {- at -} GMAIL {- dot -} COM Date: 2009-08-07 Hello all, While trying to perform some docking experiments with crystal structures into cryoEM maps, I found that some deposited EM maps are not at the right size relative to crystal structure coordinates (e.g. a ccp4 formatted deposited EM map looks smaller than a crystal structure that's supposed to represent less than 10% of the map). The problem is independent of the graphics program used (e.g. VMD, chimera, pymol all show the EM map as too small). Is there any straightforward way to get the map to the right scale, using information somehow hidden in the map header? Note that I don't know a priori by how much the EM map would have to be inflated. I'm using the SITUS package for docking - converting from ccp4 to situs, spider or any other common map format all deliver maps with the wrong size. Sincerely, Filip Van Petegem -- Filip Van Petegem, PhD Assistant Professor The University of British Columbia Dept. of Biochemistry and Molecular Biology 2350 Health Sciences Mall - Rm 2.356 Vancouver, V6T 1Z3 phone: +1 604 827 4267 email: filip.vanpetegem@gmail.com http://crg.ubc.ca/VanPetegem/ CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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