Re: [ccp4bb] DNA binding protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: AW: Disulfide bond "survival" in the presence of DTT?
From: Clemens Steegborn Clemens {- dot -} Steegborn {- at -} RUB {- dot -} DE
Date: 2009-08-11

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Subject: Practical Course for Macromolecular Crystallography M2M-2009
From: Victor Lamzin victor {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-08-11

Subject: Re: DNA binding protein
From: Lisa Mathiasen lisa {- dot -} mathiasen {- at -} IFOM-IEO-CAMPUS {- dot -} IT
Date: 2009-08-11

You can run a few µl of your sample on a native gel or agarose gel and
visualise co-purified DNA by ethidium bromide/gel red staining.

If your protein is stable at high salt concentrations (and a lot of DNA-binding
proteins are) you can use a high salt concentration (like 1 M) in the lysis
buffer.

Ion exchange is also a good way to get rid of DNA, in my experience.

Lisa

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: AW: Disulfide bond "survival" in the presence of DTT?
From: Clemens Steegborn Clemens {- dot -} Steegborn {- at -} RUB {- dot -} DE
Date: 2009-08-11

Next message:
Subject: Practical Course for Macromolecular Crystallography M2M-2009
From: Victor Lamzin victor {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2009-08-11