Re: [ccp4bb] protease cleavage sites

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: CNS v1.2 and unwanted introduction of OXT
From: "Fred {- dot -} Vellieux" vellieux {- at -} IBS {- dot -} FR
Date: 2007-03-05

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Subject: Re: MTZ to Shel-X?
From: "Ian J {- dot -} Tickle" i {- dot -} tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-03-05

Subject: Re: protease cleavage sites
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2007-03-05

PreScission protease worked great for all proteins and constructs I tested and purified. The cleavage is very specific and works in a decent range of salt conditions as well.

Sometimes, you may have to optimize digestion time and enzyme/protein ratio to get 100% or maximum-possible cleavage.

Raji

---------Included Message----------
>Hi,
>
>A non-ccp4 Q. Sorry.
>
>I would like to use a cleavable purification tag at the N-terminus/
>extracellular end of my membrane protein for purification. Before I
>start, I wonder if someone could recommend a particular protease site
>that I can engineer between the tag and my protein? How about a
>proprietary cleavage system such as the PreScission protease (GE
>Healthcare)? I would be grateful to hear success and horror stories
>in this area.
>
>Best wishes,
>
>Rene
>
>================================================
>Dr R.A.W. Frank, PhD
>Royal Commission for the Exhibition of 1851 Research Fellow
>
>Prof Seth Grant Lab / Genes to Cognition
>Wellcome Trust Sanger Institute
>Hinxton
>Cambridge CB10 1SA
>
>Work Tel: 0044 (0)1223 834244 ext. 7318
>Cell No.: 0044 (0)7870 208280
>===============================================
>
>
>
>
>
---------End of Included Message----------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: CNS v1.2 and unwanted introduction of OXT
From: "Fred {- dot -} Vellieux" vellieux {- at -} IBS {- dot -} FR
Date: 2007-03-05

Next message:
Subject: Re: MTZ to Shel-X?
From: "Ian J {- dot -} Tickle" i {- dot -} tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2007-03-05