[ccp4bb] suggestions for SIR with strong diffraction anisotropy in the derivative

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Arpwarp not recognised by latest version of ccp4
From: Sylvia Fanucchi Sylvia {- dot -} Fanucchi {- at -} WITS {- dot -} AC {- dot -} ZA
Date: 2009-08-26

Next message:
Subject: MrBUMP
From: riya doreen driya28 {- at -} GMAIL {- dot -} COM
Date: 2009-08-26

Subject: suggestions for SIR with strong diffraction anisotropy in the derivative
From: Francis E Reyes Francis {- dot -} Reyes {- at -} COLORADO {- dot -} EDU
Date: 2009-08-26

Hi all

I'm trying SIR on my crystal both are C222.

Native
Cell Dimensions: 97.66 243.45 87.62 90 90 90
Resolution: 4.3

Derivative
Cell Dimensions: 96.9100 244.66 87.61 90 90 90
Resolution: 4.7

. I plan to scale together using FHScal. Ctruncate says that my
derivative has strong diffraction anisotropy. How concerned should I
be about this at the level of phasing? Is there anything to be done
with the anisotropy issue?

Thanks

---------------------------------------------
Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder

gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D

8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Arpwarp not recognised by latest version of ccp4
From: Sylvia Fanucchi Sylvia {- dot -} Fanucchi {- at -} WITS {- dot -} AC {- dot -} ZA
Date: 2009-08-26

Next message:
Subject: MrBUMP
From: riya doreen driya28 {- at -} GMAIL {- dot -} COM
Date: 2009-08-26