[ccp4bb] Lipid Removal from Proteins

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2009-08-27

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From: aidong ahan {- at -} XMU {- dot -} EDU {- dot -} CN
Date: 2009-08-28

Subject: Lipid Removal from Proteins
From: James Stroud xtald00d {- at -} GMAIL {- dot -} COM
Date: 2009-08-28

Hello All,

I dug around on the net and found this method to remove lipids from
proteins:

"Wessel & Fluegge (1984), Anal. Biochem. 138:141-143. It´s a methanol/
chloroform precipitation and gives you a pellet that is easily
redissolved. The method was especially devised for removing lipids or
detergents, so it should be perfect for you."

-- http://www.bio.net/bionet/mm/methods/1996-December/052513.html

Is this still the preferred way? I do not want to use reagents that
are *themselves* likely to denature my protein. Has anyone tried
cyclodextrins? I'm specifically trying to strip sarcosyl. I want to do
it completely.

James

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Is there Windows version of O ?
From: lei feng spartanfenglei {- at -} HOTMAIL {- dot -} COM
Date: 2009-08-27

Next message:
Subject: weak magnet of goniometer of MAR345dtb
From: aidong ahan {- at -} XMU {- dot -} EDU {- dot -} CN
Date: 2009-08-28