Re: [ccp4bb] Cold Cabinet Vs Fridge, FPLC

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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Subject: Re: Cold Cabinet Vs Fridge, FPLC
From: "Mark J {- dot -} van Raaij" mark {- dot -} vanraaij {- at -} USC {- dot -} ES
Date: 2009-09-01

Another consideration is that you may want to run some (most)
experiments in the cold and others at higher temperatures.
We had a case of a cold-sensitive protein that could only be
effectively purified at 20 degrees Celsius - and the cabinet where we
have our FPLC in with variable temperature was very helpful here.

Mark J. van Raaij
Dpto de Bioquímica, Facultad de Farmacia
Universidad de Santiago
15782 Santiago de Compostela
Spain
http://web.usc.es/~vanraaij/

On 1 Sep 2009, at 06:31, Ho Leung Ng wrote:

> I know several labs that keep their FPLCs at room temperature. Maybe
> the cold cabinet isn't necessary?
>
>
> ho

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Post doctoral position at Karolinska Institutet
From: Gunter Schneider Gunter {- dot -} Schneider {- at -} KI {- dot -} SE
Date: 2009-09-01

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Subject: Re: citation for MOSFLM
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2009-09-01