[ccp4bb] cracking crystal

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: antibody canonical CDR structures
From: Sudharsan Sridharan sudhaarsan {- at -} GMAIL {- dot -} COM
Date: 2009-09-01

Next message:
Subject: Re: cracking crystal
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-09-01

Subject: cracking crystal
From: Fengxia Liu fengxia {- dot -} liu {- at -} GMAIL {- dot -} COM
Date: 2009-09-01

Dear All,

Could you please help me solve this problem?

I have a sensitive crystal, the mother liquor is 10% PEG4k+ 100 mM
Tris-buffer pH 8.5, crystal is big and good but very sensitive, when i put
it in cryoprotectants, cracking happened. First i used artificial mother
liquor + 25% v/v glycerol, slowly decrease to + 12.5% v/v glycerol, all
crystals cracked after immersed, finally i tried 50% mineral oil + 50%
paratone, it still cracked (4- 5 cracks, but not broken) . even this cracked
crystal can give me 3.2 angstrom diffraction, so no cracked crystal might
give me 2.0+ angstrom diffraction. Now i don't have many crystals to try so
many cryoprotectants, so anybody has experience on this? any suggestion
would be greatly appreciated.

Thank you.
Fengxiale

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: antibody canonical CDR structures
From: Sudharsan Sridharan sudhaarsan {- at -} GMAIL {- dot -} COM
Date: 2009-09-01

Next message:
Subject: Re: cracking crystal
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2009-09-01